Contact an Account ManagerQuantitative determination of lysophosphatidic acids (LPAs) in human saliva and gingival crevicular fluid (GCF) by LC-MS/MS
Summary
Type: HPLC
Application #: 126300
Detection:
Conditions: Eluent A: methanol – water (75:25, v/v) + 0.5 % formic acid + 5 mmol/L ammonium formate
Eluent B: methanol – water (99:0.5, v/v) + 0.5 % formic acid + 5 mmol/L ammonium formate
Gradient: 100 % A for 1 min, 100-0 % A in 1 min, 0 % A for 2.5 min, 0-100 % A in 0.5 min, 100 % A for 3.5 min
Flow rate: 0.5 mL/min
Temperature: 23 °C
Injection volume: 10 µL
Authors: Bathena, S.P. et al.
Source: J Pharm Biomed Anal. 2011, 10; 56(2): 402–407
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